World Drug Delivery Summit

Biography

Biography: Gagan Kaushal

Abstract

Nanoparticle based protein delivery systems are attractive for a variety of reasons. In formulating the nanoparticles for protein delivery a wide variety of harsh process conditions are used which are known to adversely affect protein stability and are mainly responsible for the uncontrolled protein release profiles. Growth hormone (GH) is a protein that is essential for growth and is used as a model protein for our studies. The adsorption characteristics of r-hGH onto Poly(D,L‐lactic‐co‐glycolic acid) (PLGA) nanoparticles were studied in the presence of competing proteins under conditions of varying solution pH. Since release of the protein from the polymer can be modulated using surface active agents such as surfactants or other proteins, a mechanistic understanding of the adsorption characteristics in presence of these agents may provide strategies for successful design of nanoparticle based delivery systems for proteins. The intent of the study was to characterize the effect of proteins of varying properties on interaction of r-hGH with PLGA surface. Knowing the nature of interactions that govern the instability of proteins at solid/liquid interfaces facilitates successful design and development of protein formulations. These studies have helped us to understand the nature of interactions that govern the adsorption process and will enable us to select a biocompatible polymer for drug delivery and modulate its drug loading. The data shows that the interaction of r-hGH on hydrophobic polymer surfaces can be modulated by the presence of competing proteins in the solution.