Mansi K Shah
Amneal Pharmaceuticals, USA
Title: Preparation, in vitro evaluation, statistical optimization and application of Caco-2 cell line for in vitro absorption mechanism of carvedilol-loaded solid lipid nanoparticles for oral delivery
Biography
Biography: Mansi K Shah
Abstract
The availability of reliable screening methods for rapid evaluation and prediction of the absorption mechanism is needed with lipid based drug delivery systems especially for solid lipid nanoparticles (SLN). Till the date, in vitro method for absorption mechanism of SLN administered orally difficiating active absorption via lymphatic uptake from the passive absorption has not been reported. Various researchers have tried methods such as intra-duodenal administration, ex-vivo with cannulation of lymphatic duct, or animal studies. However, there is no in vitro method, which can be used as a screening tool for the active absorption of SLN before performing in vivo experiments. Caco-2 cells have been widely used and accepted tool for the investigation of transport across small intestinal epithelium of many substances. The direct relationship of Caco-2 cell line with the lymphatic absorption has not been evaluated to the best of our knowledge. However, Caco-2 cell line has been used to determine the effect of lipid based excipients as formulation variable for the lymphatic delivery of therapeutic agents. Amongst the lipids evaluated, long-chain fatty acid forms lipoprotein assembly by associating with intestinal lipoproteins in the enterocytes and thereby facilitates lymphatic transport of therapeutic agents. Thus, Caco-2 cell line could have a potential application as screening tool for lipid based formulations targeted to lymphatic absorption.