Ahmed Abdelfattah Hafez Abdellatif
Azhar University, Egypt
Title: Distribution, Targeting, and Internalization of somatostatin receptors subtype 2
Biography
Biography: Ahmed Abdelfattah Hafez Abdellatif
Abstract
Somatostatin receptor subtype 2 (SSTR2) is widely expressed in normal and tumor cells, which is clinically used for diagnostic purposes. Targeting therapeutic gold and quantum dots nanoparticles (Q.G.NPs) to the SSTR2 using different somatostatin analogue such as somatostatin (SST), octreotide (OCT) and or vapreotide (VAP), would be a promising approach for certain cancer cells, since this would reduce side effects and increase drug delivery efficacy to the target site, highly over-expressing the respective receptors. The study focused on the methods of preparation and the characterization of different Q.G.NPs tagged with SST, OCT, VAP. This was followed by successful cellular uptake experiments and in vivo studies of the Q.G.NPs. The results showed a successful characterization for Q.G.NPs with SST, OCT and VAP. Cellular uptake showed highly specific interactions with cells using confocal microscopy and flow cytometry. The Q.G.NPs can be displaced from their receptor, which relates to the specificity of the interaction with somatostatin receptor. In vivo study showed a massive accumulation of Q.G.NPs in the blood as compared to the other organs, which may relate to the interaction with somatostatin receptor overexpreed on the Monocytes. In conclusion, the presented Nanoparticles can target the somatostatin receptor overexpressed on blood cells, which will serve in treating some blood disease such as Leukemia.