Qing Li
MedImmune, USA
Title: Understanding the impact of PEGylation on pharmacokinetic and biophysical properties of PEGylated diabody
Biography
Biography: Qing Li
Abstract
PEGylation has been widely used to improve pharmacokinetics of biologics. We have evaluated the effects of PEG size, shape and conjugation methods on the PEGylated diabodies. We modified diabody with PEGs of different molecular weight and shape, and applied different conjugation methods. We also measured hydrodynamic size of PEGylated diabodies with multi-angle light scattering. We found the pharmacokinetic properties of modified diabody significantly improved when Rh increased up to 6nm. In addition, PEGylation significantly reduced the non-specific binding of the diabody conjugates. Understanding the impact of PEGylation on pharmacokinetic and biophysical properties would help develop PEGylated diabody as therapeutics