Dra Ana Belén Moldes
University of Vigo, Spain
Title: GRAMICIDIN COMBINED WITH LIPOPEPTIDES PRODUCED BY A. aneurinilyticus AS A NEW COMPLEX DRUG
Biography
Biography: Dra Ana Belén Moldes
Abstract
Based on previous studies, in this work a complex drug extract produced by Aneurinibacillus aneurinilyticus is analyzed in terms of composition with the aim to study its potential interest for including it on pharmacological formulations. Methodology & Theoretical Orientation: The complex drug was extracted from A. aneurinilyticus cells growth in Tryptic Soy Broth (TSB) using phosphate buffer saline solution (PBS) followed by a dialysis process and lyophilization to remove water and salts respectively. Following the extract was analyzed by Matrix Assisted Laser Desorption/Ionization-Mass (MALDI-TOF) and Fourier Transform Infrared Spectroscopy (FTIR). Commercial Gramicidin and Surfactin (lipopeptide produced by Bacillus subtillis) were used as controls. Findings: It was observed that it is possible to obtain, from A. aneurinilyticus cells, a complex drug extract composed by Gramicidin and lipopeptides, being Gramicidin S, the predominant form of Gramicidin molecules detected in the microbial complex extract. Figure 1 shows a A. aneurinilyticus colony. Probably lipopeptides production by A. aneurinilyticus is related to the production of Gramicidin. Therefore, lipopeptides could promote the excretion of antibiotics to the extracellular media. Conclusion & Significance: Based on the results showed in this work and the microbial coexistence of Gramicidin and lipopeptides in the extracts produced by A. aneurinilyticus it should be interesting to carried out pharmaceutical assays based on Gramicidin where synthetic solubilizing and carrier agents are replaced by lipopeptides. It could be expected that lipopeptides increases the permeation and efficacy of drugs in comparison with non-natural carrier agents.