Dorcas Akinloye
Federal University of Agriculture, Abeokuta, Nigeria
Title: Prophylactic effects of Ficus exasperata N-Hexane-Ethyl Acetate Extract against CCl4 - Induced NF-kB/TNF-α expressions in Female Wistar Rats
Biography
Biography: Dorcas Akinloye
Abstract
The study aims to investigate the protective effects of Ficus exasperata n-hexane ethyl acetate extract against carbon tetrachloride (CClâ‚„)-induced tissue damage in female Wistar rats; using specific activities of alkaline phosphatase (ALP), gamma-glutamyl-transferase (GGT), and 5’ nucleotidase (5’NTDase) along with relative gene expression of nuclear factor-kappa B (NF-kB) and tumor necrosis factor-alpha (TNF-α) as indices. Thirty-six female Wistar rats were divided into six groups (A, B, C, D, E, F) of six rats per group. First, groups A and B were pre-treated with olive oil. Next, groups C, D, E, and F were pre-treated with Vitamin E (100 mg/ kilogram bodyweight (kg bwt)), extract (100 mg/ kg bwt), extract (200 mg/ kg bwt), and extract (200 mg/ kg bwt) respectively for fourteen days. Then, CClâ‚„ single dose injection (intraperitoneally) was given to rats in groups B, C, D, and E. Ficus exasperata n-hexane ethyl acetate extract pre-treatment was able to protect against CClâ‚„-induced toxicity by preventing the overexpression of NF-kB and TNF-α gene levels along with protection of membrane-bound (ALP, GGT, and 5’NTDase) enzymes linkages into the serum. In conclusion, Ficus exasperata n-hexane ethyl acetate extract could be used to prevent or manage inflammation and membrane dysfunctions.