Day 1 :
Keynote Forum
Yalew Molla
Debre Markos University, Uk
Keynote: Evaluation of antimalarial activity of the crude extract of stem bark of Combretum molle against Plasmodium berghei in mice
Biography:
Yalew Molla has completed his Bachelor Degree in Pharmacy at the age of 23 years from Jimma University in June 2007. HE has completed his MSc at the age of 31 years from Addis Ababa University in December 2015. He is the coordinator of Pharmacology units in Pharmacy Department. He has teaching and reseacher experience for about four years after garaduated from Addis Ababa University. He has published more than 5 papers in reputed journals. Yalew Molla is very energetic, committed and industriousness individual to achieve towards the end of his goal.
Abstract:
People have a practice of treating malaria using different traditional medicinal plants like Combretum molle without scientific validations. Therefore, the aim of this study was to evaluate the antimalarial activity of the hydroalcoholic stem bark extract of Compertum molle in mice. The mice were randomly divided into five groups (n=5). Group-I mice were treated as negative control and received 3% Tween 80, group-II was treated with 25mg/kg Chloroquine (taken as positive control) and group III, IV & V were treated with 100mg/kg, 200mg/kg and 400mg/kg of the crude extract respectively. Data were analyzed by SPSS version-21 using one-way ANOVA followed by Tukey post hoc analysis. In the chemosuppressive test, the parasitic suppression effect of the crude extract at all doses was found to be significant (P<0.05) as compared to the negative control in a dose dependent pattern. In addition, the 400mg/kg and the 200mg/kg dose of the crude extract had prolonged the survival of mice as compared to the negative control (p<0.05). In the curative test, the average parasitic level of those mice treated by the three doses of the crude extract had been shown to be significantly suppressed at D5, D6 and D7 of treatment as compared to the negative control (p<0.001). The findings of present study had supported the folklore use of the leaves of Combretum molle in the treatment of malaria. Therefore, further fractionation, and characterization of crude extract is necessary to identify the responsible lead compound/s with the potential of antiplasmodial activity.
- Advanced Drug Delivery Systems, Drug Delivery Technologies, Nanotechnology for better Drug Deliverability
Chair
Yalew Molla
Debre Markos University
Session Introduction
Julio César Fernández-Travieso
1National Centre for Scientific Research, , Havana, Cuba
Title: A THERAPEUTIC OPTION FOR THE MANAGEMENT OF SYMPTOMS IN PATIENTS WITH OSTEOARTHRITIS
Biography:
Julio César Fernández-Travieso, 1National Centre for Scientific Research, , Havana, Cuba
Abstract:
Abstract
Objective: Investigate and demonstrate the benefits of Abexol treatment on symptoms in patients with osteoarthritis.
Methods: Six randomized clinical studies were conducted with Abexol on symptoms in patients with osteoarthritis: two to double blind, placebo controlled six and eight weeks of treatment, one comparative with Lyprinol, another comparative with Prevenox and its combined therapy, and two open, comparative with Chondroitin sulfate/Glucosamine three and six month of treatments. The primary outcome was the reduction of the total WOMAC score. Secondary outcomes included WOMAC pain, stiffness and function scores and VAS score. The reduction of consumption of analgesics was a collateral outcome. In all studies the data were analysed as per the Intention to treat approach.
Results: Abexol treatment produced a documented clinical improvement in patients with osteoarthritis, which was reflected in a significant improvement in pain, stiffness, physical activity and overall symptomatic status, through the total WOMAC score and score of pain of the VAS scale, with an efficacy superior to Lyprinol and comparable to Prevenox and Chondroitin sulfate/Glucosamine. Abexol treatment significantly reduced the consumption of analgesics in these patients. The treatments were safe and well tolerated.
Conclusions: It is concluded that according to the efficacy and safety shown by Abexol in the treatment of patients with osteoarthritis, Abexol could be an alternative for the management of these patients, mainly in those patients who have contraindicated treatment with non-steroidal anti-inflammatories and paracetamol.
Keywords: osteoarthritis symptoms, abexol, lyprinol, chondroitin sulphate/glucosamine, WOMAC score, VAS score.
Rahmat Asfiya
Indian Institute of Science, Dept. Of Organic Chemistry, Bangalore, INDIA
Title: Efficacious anticancer drug delivery mediated by a short biocompatible peptide sequence targeted for colon cancer
Biography:
Ms. Rahmat Asfiya is a Ph.D student, working under supervision of Prof. Santanu Bhattacharya, Dept. of Organic Chemistry, Indian Institute of Science, Bangalore, India. She is a Senior Research Fellow (SRF) and working in the field of chemical biology. Her expertise and passion is in improving of anticancer therapeutics delivery effectively and efficiently to the target site by means of delivery vehicles. So far she has been worked in development of some new vehicle based of short peptide, gold nanoparticles and lipid based molecules.
Abstract:
A tetrapeptide and octapeptide sequence were synthesized to evaluate their efficiency towards the delivery of a common anticancer drug, doxorubicin. Initially we investigated the self-organization pattern of both of the peptide sequences where they exhibited concentration dependent self-assembling behaviour. Particularly the tetrapeptide showed vesicular morphology at low concentration and fibrous morphology at high concentration. The observations were corroborated from both the atomic force and transmission electron microscopic analysis.
Importantly, the self-assembled nanostructures were quite sensitive towards the pH of the medium. The vesicles, made up of either the tetra or octapeptide sequences, were stable at lower pH (~pH 5) of the medium, but ruptured rapidly at physiological pH (~pH 7.4). This pH-sensitive nature of the vesicles was then tested for the delivery of one of the widely used anticancer drug, doxorubicin (DOX), where the octapeptide showed its proficiency not only towards the encapsulation but also towards the sustained release of DOX. The drug release profile followed the pattern of pH 7.4 > pH 6 > pH 5.
The vesicles were further coated with derivatives of polyethylene glycol based lipids to increase the blood circulation time and serum stability of the DOX loaded nanoparticles. The in vitro efficacy of the system was monitored in stomach cancer cell line, AGS, and colon carcinoma cell line, CT26. The cellular internalization of DOX was found to be better when the drug was delivered by the PEGylated octapeptide system as compared to the tetrapeptide system and drug alone. The observation was confirmed from the flow cytometric, fluorescence and confocal microscopic analyses.
Abdeen Omer
Ministry of Health and Social Welfare, Khartoum, Sudan
Title: Regulation and control of the importation, exportation, manufacture, advertisement, distribution, sale and the use of medicines, cosmetics, medical devices and chemicals
Biography:
Abdeen Mustafa Omer (BSc, MSc, PhD) is an Associate Researcher at Occupational Health Administration, Ministry of Health and Social Welfare, Khartoum, Sudan. He has been listed in the book WHO’S WHO in the World 2005, 2006, 2007 and 2010. He has published over 300 papers in peer-reviewed journals, 200 review articles, 7 books and 150 chapters in books.
Abstract:
Worldwide there are different systems for providing pharmacy services. Most countries have some element of state assistance, either for all patients or selected groups such as children, and some private provisions. Medicines are financed either through cost sharing or full private. The role of the private services is therefore much more significant. Nationally, there is a mismatch between the numbers of pharmacists and where are they worked, and the demand for pharmacy services. The position is exacerbated locally where in some areas of poor; there is a real need for pharmacy services, which is not being met and where pharmacists have little spare capacity. Various changes within the health-care system require serious attention be given to the pharmacy human resources need. In order to stem the brain drain of pharmacists, it is, however, necessary to have accurate information regarding the reasons that make the pharmacists emigrate to the private sector. Such knowledge is an essential in making of informed decisions regarding the retention of qualified, skilled pharmacists in the public sector for long time. There are currently 3000 pharmacists registered with the Sudan Medical Council of whom only 10% are working with the government. The pharmacist: population ratio indicates there is one pharmacist for every 11,433 inhabitants in Sudan, compared to the World Health Organisation (WHO) average for industrialised countries of one pharmacist for 2,300 inhabitants.
Muhammad Jehangir
Senior Manager Quality Control and Validation Novamed Group
Title: Pharmaceutical Impurity Analysis of Raw Materials and Final Product by using analytical techniques
Biography:
Muhammad Jehangir has 13 years diversified experience of Quality Control, Quality Assurance, Registration Affairs, Product development and Pharmaceutical manufacturing, Process Planning, Method development, Method validation, Statistical Methodology, Process & Cleaning Validation, Equipment Validation etc. Certificate Courses on cGMP, cGLP, Process Validation, CTD Documents, ISO 9001:2008, 13485-2003,14001-2004 have strong scientific, analytical, statistical, managerial and training skills. Currently he is working as a Senior Manager Quality Control and validation for Novamed Pharmaceuticals.It is toll manufacturing oriented company, manufacturing of companies like Getz Pharma, ICI, SEARLE, Macter, Ray, and for Sanofi-Aventis. He is also looking after the Quality of Novamed Healthcare, the nutraceutical and cosmeceutical manufacturing plant
Abstract:
The evaluation of pharmaceutical raw materials and finished products for impurities and degradation products is an essential part of the drug development and manufacturing testing process. Additionally, toxicological information must be obtained on any drug-related impurity that is present at a concentration of greater than 0.1% of that of the active pharmaceutical ingredient (API). In pharmaceutical QC and manufacturing, impurity analysis has traditionally been performed by HPLC with UV, PDA, or MS detection. As it is essential to detect and measure all of the impurities in the sample, it is necessary to have a high resolution separation process. This usually involves long analysis times resulting in low throughput. As candidate pharmaceutical compounds become more potent and are dosed at lower and lower levels, ever more sensitive assays are needed to detect and measure impurities. The low throughput of HPLC can become the rate-limiting step in product release testing or process evaluation. Since much of the process of impurity identification involves the coupling of LC to sophisticated MS, any reduction in analysis time will result in a more efficient use of these significant investments. Analytical technology advances such as UPLC and UPC offer significant improvements in throughput and sensitivity, with benefits to the process of product release and identification of drug-related impurities. The most characteristic feature of the development in the methodology of pharmaceutical and biomedical analysis during the past 25 years is that HPLC became undoubtedly the most important analytical method for identification and quantification of drugs, either in their active pharmaceutical ingredient or in their formulations during the process of their discovery, development and manufacturing.